(1.College of Environment, Hohai University, Nanjing 210098, China;2.Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China;3.College of Oceanography, Hohai University, Nanjing 210098, China)
In this study, the ciprofloxacin (CFC) and copper (Cu) were selected as the target pollutants, indoor culture experiments were designed, and the interaction relationship between microbial communities and antibiotic resistance genes (ARGs)under ciprofloxacin and copper co-contamination in river sediments was explored using high throughput 16S rRNA sequencing and quantitative PCR. The results showed that the ARGs abundance, microbial community composition and functions were significantly different among different experimental groups (CFC contamination, Cu contamination and co-contamination). The total relative abundance of ARGs of each sample showed an increasing trend first, and then it decreased at the end of culture experiment. Co-occurrence network analysis suggested that intI 1 was associated with 3 types of ARGs and 8 bacterial communities, regarded as a bridge between ARGs and microbial community. Meanwhile, the bacteria that belonged to Proteobacteria, Acidobacteria, Firmicutes, Bacteroidetes and Chloroflexi was considered as the potential ARGs hosts in the aquatic environment. Based on the partial redundancy analysis, the microbial community had the highest overall interpretation rate (49.77%) for the change of ARGs abundance among MGEs, microbial communities, pollutants and other factors. At last, the path analysis showed that CFC and Cu pollutants indirectly affect the spread and diffusion of ARGs mainly through microbial communities.
李轶,胡童,王琳琼,等.环丙沙星和铜复合污染下河流底质微生物群落与抗生素抗性基因的交互关系[J].河海大学学报(自然科学版),2022,50(6):75-84.(LI Yi, HU Tong, WANG Linqiong, et al. Interaction relationship between microbial communities and antibiotic resistance genes under ciprofloxacin and copper co-contamination in river sediments[J]. Journal of Hohai University (Natural Sciences),2022,50(6):75-84.(in Chinese))Copy