Abstract:According to the characteristics that there are high content of bio-refractory organic matters in the coking wastewater and the changes of water quality and water quantity during actual production are great, the authors of this paper mainly studied the treatment of wastewater containing phenol by high efficient strains. four highly efficient degradation bacteria strains which take phenol as the sole carbon source were obtained by means of domestication, isolation and screening to the activated sludge in the aeration tank, a biological treatment device, of the water treatment plant in a coking factory. Those four highly efficient degradation bacteria strains were identified and its degradation characteristics were studied. The results show that the strain h32a2, b31B, h31A and b41a are identified as Pseudomonas sp; Phenol content was measured by brominating volumetric method. The best condition of strain degradation was determined as follows: the degradation temperature was 32℃, the pH value is 7. 5, the strain incubation time was 16h, the inoculation density was 1%, and the maximum phenol degradation rate of the strain h32a2 reached 90. 55%. We can know that the degradation effect of combined four highly efficient degradation bacteria strains is the best from the degradation characteristics of single and combined strain. The treatment of wastewater containing phenol by mixed high efficient phenol-degradation bacteria was feasible.