In this study, the drinking water barrels made of polyethylene terephthalate(PET)and polycarbonate(PC), which were reused 0, 5, 20, and 40 times, respectively, were selected and compared with the unbarrelled pure water, and the methods of liquid chromatography-mass spectrometry and the atomic fluorescence spectrometer(AFS)were adopted to detect the concentrations of bisphenol A(BPA)and antimony(Sb)in the barrels, in order to evaluate the influences of the number of reuses and the preservation time of the two kinds of water barrels on the exudation of harmful substances. The results show that, with the increase in numbers of reuses and the extension of preservation time, the concentrations of Sb in the PET barrels and the concentrations of BPA in the PC barrels had a significantly increasing trend. In general, the number of reuses of the barrels was the main factor affecting the Sb and BPA exudation. The detected values of Sb and BPA exudation all increased significantly with the increase in numbers of reuses and the extension of preservation time. In normal operating conditions, the concentration of BPA was far less than the values recommended by WHO, EFSA, and EPA, meaning that it was within the safe range. However, the concentration of Sb would probably exceed the value recommended by Standards for Drinking Water Quality(GB5749-2006).