This study conducted a three-year monthly survey of zooplankton and environmental factors in typical Dazong Lake from January 2017 to December 2019, in order to explore the succession characteristics and driving factors of zooplankton community in Dazong Lake and comprehensively evaluate its water quality after the stable operation of water delivery project. The results show that 49 species of zooplankton were identified in Dazong lake during the survey, including 33, 9 and 7 species of rotifers, cladocerans and copepods respectively. The interspecific connectivity statistics show that the dominant species in Dazong Lake showed a significant positive correlation overall, and the correlation increased year by year, which meant the zooplankton population structure tended to be stable. Average density was 623.17 per liter, showing a downward trend, and the average biomass was 1.63 mg/L, showing an upward trend. Compared with rotifers, the proportion of cladocerans and stilts gradually increased. The results of RDA redundancy analysis show that chlorophyll a, total nitrogen and water temperature were important factors affecting the succession of zooplankton species in Dazong Lake. The organic pollution of Dazong Lake was evaluated by Shannon-Wiener diversity index, Margalef richness index and Pielou evenness index. The results were low pollution and the B/Tindex was consistent with the results of TLI eutrophication evaluation, thus Dazong Lake was in a medium eutrophic state. Zooplanktons have a good response to the changes of environmental factors in Dazhong Lake, which can be applied to the evaluation of water ecological status in the lake area.