The organizational culture of water project management units has a significant impact on their operation and management. The current evaluation status of the organizational culture of water project management units is elaborated, and the necessity of establishing the relevant evaluation index system is analyzed. The dimension division results of the organizational culture by Dension and Zhang Shijing are introduced. On this basis, the evaluation index system for the organizational culture of water project management units is established by regarding the consistency, participation, adaptability and mission of the organizational culture as index grade I and core values, team consciousness, organization consciousness, innovation awareness, social responsibility, employee awareness and objective vision as index grade II. The determination of the weights of evaluation indices of organizational culture of water project management units is briefly introduced.