The regional water resource carrying capacity is of great importance to the eco-environmental construction and socio-economic development. In the light of the principles for establishment of evaluation system of water resource carrying capacity, 3 sub-systems of water resources, socio-economy and eco-environment are listed for the development of Yancheng City. A fuzzy comprehensive evaluation model for water resource carrying capacity is established, and it is applied in Yancheng City. The results indicate that the development and utilization of water resources in Yancheng City has reached a considerable scale under the existing economic and technological conditions. The potential of water resources is relatively small, and the contradiction between water supply and demand is obvious. Some measures are proposed to improve the water resource carrying capacity.