Abstract:The port is an important node connecting the domestic road network and the world, and it has a special position in the global logistics chain. With the continuous development of China’s economy and the improvement of its opening to the outside world, higher requirements are put forward for the construction and management of ports, thus accelerating their inter-generational replacement. The connotation and characteristics of the fifth generation port are theoretically put forward, and the realization path of high-quality development of the fifth generation port is discussed based on the practical experience of Shenzhen Port. The construction of Guangdong, Hong Kong and Macao Bay area and the demand of port transformation and upgrades promote Shenzhen Port to take the leading role in carrying out the practice and exploration of the fifth generation port. Shenzhen Port has made certain achievements in port-city interaction, smart port, green port, etc. , and its high-quality development has achieved initial results.