There are many stakeholders involved in the comprehensive reform of agricultural water prices, but the policy deviates from their interests, which is the key issue that hinders the progress of the reform. Through stakeholder theory analysis, a theoretical system of agricultural water price stakeholder research is initially established in the paper. The classification, interest relations and interest demands of agricultural water price stakeholders are clarified separately, and the mechanism of stakeholders’ influence on agricultural water price is sorted out clearly. At the same time, we also use the “Mitchell” scale and the expert scoring method to quantitatively assess the interests of each party in the reform. The results of the study showed that: Agricultural water price stakeholders have different interests and demands, and coordinating their interests is conducive to breaking through the dilemma of reform; The results of the benefit evaluation are expressed as “Government > Farmers > Social institutions”, “Suppliers > Users > Support and guarantee parties”, so the main responsibility of governments should be emphasized in the reform; In order to guide farmers, farmers’ water associations and social institutions to participate and cooperate in the reform, the government should provide reasonable policy support and publicity guidance.