In order to recover phosphorus from macrophytes fermentation solution effectively, the iris(Iris sibirica L. )and the Hydrocotyle vulgaris were chosen for carrying out fermentation experiments. The efficiency and purity of phosphorus recovery in fermentation broth under different pH values were analyzed and the possibility of phosphorus recovery from macrophytes fermentation solution with the method of hydroxyapatite crystallization was explored. The results showed that it was suitable to recover phosphorus by hydroxyapatite(HAP)crystallization when the ration of Ca to P were 2. 7 and 10 and the ratio of Mg to P were 1. 4 and 1. 6 in the fermentation solutions of Iris sibirica L and Hydrocotyle vulgaris. Different macrophytes fermentation solutions displayed similar process of phosphorus recovery at adjusted pH. The phosphorus recovery rate of iris and H. vulgaris fermentation solutions reached 89% and 91%, respectively when pH value was 8. 5 and hydroxyapatite precipitates had good settleability. The results of X ray fluorescence(XRF)analysis indicated that when pH was 8. 5, the P₂O₅ mass fraction in the precipitates exceeded 25% and the main component was hydroxyapatite. When pH was adjusted beyond 8. 5, the calcium carbonate content in the precipitates would increase with the rise of pH, which would affect the purity of the hydroxyapatite in the precipitates. Therefore, 8. 5 was the suitable pH value for phosphate recovery.