Aiming at the ecological water demand of delta wetland, the water demand of estuarine and offshore fish, and sediment transport, according to the characteristics of different water demand targets, based on the habitat simulation model and functional linear regression model, the effects of runoff process on the habitat and biomass of biological community in delta wetland and estuary area, and the effects of incoming sediment coefficient on the sediment transport were calculated respectively. The calculation of consumptive water demand was followed the additive principle and non-consumptive water demand was followed the maximum principle, the water demand and discharge process that meet the multi-objective of delta wetland ecological water demand, estuarine and offshore fish water demand, and sediment transport were put forward. The results show that regulation of the discharge process according to time period can better meet the demand of different guarantee objectives. Certain discharge can be provided in March, and the minimum, suitable and maximum discharge from April to May are 456. 91 m³/s, 634. 04 m³/s, 1 107. 38 m³/s, respectively, while in other months are 146. 91 m³/s, 559. 79 m³/s and 1 107. 38 m³/s, respectively. The minimum, suitable and maximum water demand in the Yellow River Estuary are 71. 05 billion m³, 181. 51 billion m³ and 349. 85 billion m³, respectively. By ensuring the total ecological water demand in the year and maintaining the specific discharge during the critical growth period of specific organism, the habitat quality of delta wetland, estuarine and offshore areas can be effectively improved, so as to provide theoretical support for the ecological protection and restoration of the Yellow River Estuary.