To reveal the effect of different water and carbon managements on the N2O production in the paddy soil, a combined watersaving irrigation with the straw returning and organic fertilizer application was adopted to observe the profile distribution characteristics of N2O concentration in paddy soil with the microlevel technology under different water and carbon managements. Results show that within 16 days after fertilization, N2O concentration in the controlled irrigation paddy soil is lower than the conventional irrigated soil in different depth, from 8 day the controlled irrigation paddy soil is significantly higher than the conventional irrigated soil (p<0.05), and the law is basically same under two carbon managements. N2O concentration in the shallow layer (01cm) of the controlled irrigation paddy soil is not significantly affected by the straw returning and the organic fertilizer application, while in the deeper depth (13 cm), the straw returning is significantly higher than the organic fertilizer application (p<0.05). The effect of two carbon managements on N2O concentration in the conventional irrigation paddy soil is not obvious. N2O concentration of organic fertilizer application under the controlled irrigation is lower than others, after the tiller fertilizer, the mean concentration of N2O in CM is 35.1% and 24.8% lower than it in CS and FM (p<0.05), and CM is slightly lower than FS. After the panicle fertilizer, the mean concentrations of N2O are CM is 45.5%, 33.7% and 18.8% lower than CS, FS and FM (p<0.05).