In order to study the deterioration law of the bond performance of adhesive-concrete interface, the deterioration tests of adhesive-concrete specimens were designed under the environments of freshwater, coupling of freshwaters and sustain load, and freeze-thaw cycles. Considering two kinds of concrete strengths and two kinds of adhesive in the tests, a total of 100 sandwiched beam specimens containing adhesive-concrete interface were prepared. After the deterioration treatment, the specimens were experimented by four-point bending test, and the failure load, failure mode and load-displacement curves at different time were measured. Based on test results, the deterioration law of bond performance of the adhesive-concrete interface was analyzed. The test results showed that the influence of freshwater environment on the adhesive-concrete interface was negligible, the deterioration effect under coupled environment of freshwater and sustain load was more significant, and the ultimate load capacity of specimens decreased by 41. 2% after 42 days. Besides, the deterioration effect of freeze-thaw cycles was not significant after 25-times cycles, but the ultimate load capacity of the specimens decreased by 28. 3% after 50-times cycles. The failure mode of specimens under various deterioration conditions was adhesive-concrete mixed failure, and the load-displacement curves showed that this kind of failure was brittle failure.