To have a deep insight into the turbulence characteristics of water flow around a perforated siltation-promoting plate, the change law of the turbulence characteristics before and after the plate was analyzed through an indoor generalized water tank test. The three-dimensional instantaneous flow velocity on the typical sections before and after the perforated silting-promoting plate was measured by acoustic Doppler velocimetry (ADV), and the distribution law of time-averaged flow velocity, turbulence intensity, Reynolds shear stress and turbulent kinetic energy at typical sections were also analyzed. The test results show that there is a recirculation zone in the area near the plate back, and the recirculation zone corresponds to the position of the grille of the perforated siltation-promoting plate. The relative turbulent intensity, Reynolds shear stress and relative turbulent kinetic energy behind the plate are significantly larger than those in front of the plate. The plate has a great influence on the flow in the area near the plate back. With the increase of the distance from the perforated siltation-promoting plate, the recirculation phenomenon disappears. The change of the flow velocity tends to be steady, the relative turbulent intensity decreases and finally tends to be stable, and the relative turbulent kinetic energy decreases with a decreased reduction rate. The Reynolds shear stress at the xOy plane close to the near-plate area is greater than that at the yOz plane, and the Reynolds shear stress at the xOy plane adjacent to downstream is close to that at the yOz plane.