In order to promote the application of aeration technology in alleviating the influence of supersaturated dissolved oxygen (DO),the release tests of supersaturated DO under non-aeration and aeration conditions were carried out. The impact of aeration on the dissipation process of supersaturated DO and its related laws were studied, and the release process and release coefficient of supersaturated DO under different aeration conditions were analyzed. The experimental results show that the time required for DO saturation to dissipate from about 170% to about 105% under aeration conditions is less than 11 min, while the time required under no aeration conditions is at least 240 min, indicating that aeration can significantly promote the dissipation of supersaturated DO. The supersaturated DO release coefficient increases with the increase of aeration rate, decreases with the increase of aeration depth, and shows a strong negative correlation power function relationship with aeration aperture. According to the release law of supersaturated DO, the quantitative relationship between release coefficient and aeration conditions was established. The quantitative relationship between release coefficient and aeration conditions, model size and fluid parameters was established by dimensional analysis. By comprehensively analyzing the two quantitative relationships, it can be concluded that the latter has smaller root mean square error and average absolute error, higher correlation coefficients, and easier parameter acquisition, which shows that the latter is more applicable in practice.