The wetted perimeter method, Tennant method, Tessman method and month-by-month minimum ecological runoff calculation method were used to calculate the ecological flow respectively. The flow required for fish breeding and spawning was applied to verify the rationality of the four methods. The results show that the Tennant method, Tessman method, and month-by-month minimum ecological runoff calculation method can ensure the minimum flow required for the fish breeding and spawning at the Zumuzu cross section and all the four methods can ensure the minimum flow required at the Dajin section. Tennant method is recommended from March to August and the wetted perimeter method is suggested to use from September to February. The minimum ecological flow process can meet the requirements of fish and habitat protection, and has the smallest impact on human water intake, which can provide reference for the determination of ecological flow.