Indicators for aquatic ecoregions at levels I and a level Ⅱ are defined according to the driving force-pressure-state-impact-response (DPSIR) framework, and an indicator system is established on the basis of assessment of the relationship between environmental and aquatic ecological components, and the spatial heterogeneity of the environmental components. A combined zoning method moving from top to bottom and from bottom to top is used for zoning of aquatic ecoregions at levels I and Ⅱ in the Liaohe Basin. The Liaohe Basin is divided into four aquatic ecoregions at level I and eight aquatic ecoregions at level Ⅱ. The features of each ecoregions are described. It is pointed out that subjective factors still exist in the zoning process of aquatic ecoregions in the Liaohe Basin, especially in determining the boundary of ecoregions at level Ⅱ.