To explore resistance characteristics and calculation methods of resistance parameters in uniform steady open channel flow with a suspended flexible curtain group(SFCG), 92 sets of flume experiments were carried out with different curtain spacing, curtain heights, and flow conditions. The hydraulic gradients along the flume were obtained through curve fittings of water surfaces, and the drag coefficient Manning roughness and Darcy-Weisbach friction factor were studied. It is shown that the mutual interference among the SFCG would almost disappear under the critical curtain spacing. The SFCG significantly exhausts flow energy and increases resistance, and the drag coefficient hardly changes in different turbulent flow conditions with the range from 1. 60 to 2. 05. The Manning roughness is mainly related to the relative height and arrangement density of SFCG, and a modified calculation method for Manning roughness is proposed. In addition, the Darcy-Weisbach friction factor can be obtained from a fitting formula depending on relative height, distribution density of SFCG, and Reynolds number.