In this paper, the characteristics of nitrogen loss with runoff under different land use patterns in Huashan hydrological experimental watershed are studied. Based on the data of sample measurement of nitrogen mass concentrations from October, 2012 to September, 2013 in Huashan hydrological experimental watershed, the characteristics of nitrogen loss with runoff are analyzed. The results show that, there are higher total nitrogen mass concentration and nitrate nitrogen mass concentration in the runoff of the large proportion of the forest catchment as well as higher ammonia nitrogen mass concentration in the runoff of the large proportion of the farmland catchment. The mass concentration of total nitrogen highly correlates with the mass concentration of nitrate nitrogen in catchments with a higher proportion of forest, while the mass concentration of total nitrogen highly correlates with the mass concentration of ammonia nitrogen in catchments with a higher proportion of farmland. For the nested subcatchments the mass concentration of nitrate nitrogen decreased along the river channel downstream with the increase of catchment area and the reduction of forest coverage; in nonfertilization seasons, the mass concentration of total nitrogen decreased along the river channel downstream with the increase of catchment area and the reduction of forest coverage,and the mass concentration of ammonia nitrogen has a small change, while in fertilization seasons, the mass concentration of total nitrogen increased with the increase of the mass concentration of ammonia nitrogen. The study found that,in the catchment of the Junshipai section and the Wangyingzuozhi section, the proportion of forest is as high as more than 9992％ and human activities are quite limited, however, the mass concentration of total nitrogen in these sections are 2830mg/L and 4039mg/L respectively, being 142~251 times and 202~357 times higher than the one in the catchment of Zhuyuangou section and Xinba section respectively, where the proportion of farmland is 3959％ and 3563％ respectively. This fact is inconsistent with the common knowledge of people, which is worth further studying.