Solid-phase extraction followed by high-performance liquid chromatography and tandem mass spectrometry were used to analyze the characteristics of the concentrations of five kinds of antibiotics(14 species)in three typical drinking water sources in a city in Jiangsu Province. The results show that the concentrations of the antibiotics ranged from ND to 52. 7 ng/L, and the detectable rate ranged from 0 to 78%. The sulfadiazine, sulfamethoxazole, chlortetracycline, and ofloxacin were detected most frequently, and the sulfonamides were found to have the highest concentrations. On the whole, the concentrations of antibiotics in the water sources of this study were lower than those in surface water reported in other studies in China and elsewhere. Moreover, the concentrations of antibiotics were influenced by the change of seasons and the types of water sources. Generally, the concentrations of antibiotics were ranked in the descending order, as follows: normal season(188. 3 ng/L), dry season(57. 2 ng/L), and wet season(8. 9 ng/L); and they had higher values in the source area of Taihu Lake than in the source area of the Yangtze River.