The removal efficiency of three kinds of tetracyclines(TCs)including tetracycline(TC), oxytetracycline(OTC), and chlortetracycline(CTC)from wastewater in the vertical-flow constructed wetlands(CWs)were investigated under the conditions of two different carbon sources(i. e. , glucose and sodium acetate). The results show that both glucose and sodium acetate were highly efficient in the removal of TCs in the CWs. The removal rates of TCs were enhanced with the increase of COD concentration with the same carbon source. When the COD concentration was greater than 400 mg/L, the removal rates of TCs were above 90%. The removal process of TCs by vertical-flow constructed wetland included two stages: during the first two hours, TCs concentrations had a sharp decrease mainly due to the adsorption of the wetland substrate, and during the following 22 hours, the TCs concentrations continued to decline slowly. The improvement effect of sodium acetate and glucose on the TCs removal rate mainly occurred in the second stage after 2 hours. It is considered that co-metabolism occurred in the CWs, which promoted the further degradation of TCs adsorbed to the wetland substrate.