The effluent from the secondary sedimentation tank of the sewage treatment plant was flocculated with the plant tannin flocculant (TGCC-A) modified by dimethyl diallyl ammonium chloride (DMDAAC). The removal effect of TGCC-A on intracellular antibiotic resistance genes (iARGs) and extracellular antibiotic resistance genes (eARGs) in sewage and the impact on ARGs abundance in subsequent sewage storage was investigated. The results show that the average log reduction of 8 intracellular target genes in raw water by 9mg/L TGCC-A and 7mg/L PAC are 103.82 copies/mL and 103.92 copies/mL, respectively, and the average log reduction of extracellular target genes were 102.27 copies/mL、102.89 copies/mL, respectively. The abundance of the extracellular form of the same ARGs was greater than that of the intracellular form in the water samples treated by the two flocculants, and the abundance of 8 target genes basically remained at the immediate abundance after flocculation during the subsequent 5 days of storage. TGCC-A has good removal and control effect on ARGs in secondary effluent of sewage plant.