为了对应用生物膜进行湖泊的生态监测与评价提供理论依据，通过微宇宙试验研究了不同浓度梯度养鸭废水对生物膜生物量和胞外酶活性的影响。结果表明:养鸭废水会显著增加生物膜的生物量；在短期暴露中，生物膜的生物量与养鸭废水浓度线性相关；而在长期暴露中则符合Logistic方程，养鸭废水对生物膜生物量的促进具有饱和效应。养鸭废水对生物膜胞外酶活性的效应受暴露时间的影响:短期暴露(24 h)时养鸭废水会促进碱性磷酸酶、β-葡萄糖苷酶和亮氨酸肽酶活性的增加，3种酶活性均随养鸭废水浓度的升高而呈对数增加；长期暴露(60 d)时，养鸭废水对3种酶活性均产生抑制作用，酶活性随养鸭废水浓度升高而呈对数降低。
In order to provide a theoretical basis for ecological monitoring and evaluation of lakes using biofilm, the effects of duck wastewater with different concentration gradient on the biomass and extracellular enzyme activities of biofilm were studied by microcosmic experiment. The results showed that duck wastewater significantly increased the biomass of biofilm. In short-term exposure, the biomass of biofilm(chlorophyll a and ash-free dry weight )was linearly correlated with the concentration of duck wastewater, while in long-term exposure, it was in accordance with Logistic equation, which indicated that the duck wastewater had saturation effect on the promotion of biofilm biomass. The effect of duck wastewater on extracellular enzyme activity of biofilm was affected by exposure time. The activities of alkaline phosphate(APA), glucosidase(GLU)and leucine peptidase(LAMP)were increased by short-term exposure(24 h). The activities of these three enzymes increased logarithmically with the increase of the concentration of duck wastewater. While in the long-term exposure(60 days), the activity of three kinds of enzymes were inhibited by duck wastewater, which decreased logarithmically with the concentration of duck wastewater.
马牧源,崔丽娟,张曼胤,等.白洋淀养鸭废水对生物膜生物量和胞外酶活性的影响[J].水资源保护,2020,36(1):89-96.(MA Muyuan, CUI Lijuan, ZHANG Manyin, et al. Effects of duck wastewater from Baiyangdian Lake on biofilm biomass and extracellular enzyme activities[J]. Water Resources Protection,2020,36(1):89-96.(in Chinese))复制